A section of human DNA is inserted into a bacterial plasmid, which is inserted into a bacterium. The bacterium synthesizes protein, but nothing like the human cell. Which of the following is the most likely explanation for this phenomenon?
B is the correct answer. Most of the nucleotide sequences in human DNA are non-coding. If these non-coding sections, or introns, are included in the plasmid, they will also be turned on and begin coding for amino acids, producing a completely different protein. Although the plasmid is, in a sense, artificially mutated by the insertion of the human DNA, this does not explain the different protein synthesis. Choice D is incorrect because the inserted DNA is spliced into the plasmid by an enzyme.
B is the correct answer. Most of the nucleotide sequences in human DNA are non-coding. If these non-coding sections, or introns, are included in the plasmid, they will also be turned on and begin coding for amino acids, producing a completely different protein. Although the plasmid is, in a sense, artificially mutated by the insertion of the human DNA, this does not explain the different protein synthesis. Choice D is incorrect because the inserted DNA is spliced into the plasmid by an enzyme.